Bpsbioscience/Anti–EZH2 monoclonal antibody/25222/50 µg
商品编号：
25222
品牌：
bpsbioscience
市场价：
¥7500.00
美元价：
4500.00
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
HostSpecies:Mouse
Clonality:monoclonal
Isotype:IgG1
Background:
EZH2(UniProt/Swiss–ProtentryQ15910)isahistone–lysinemethyltransferasewhichmethylatesLys–9andLys–27ofhistoneH3,leADIngtotranscriptionalrepression.Itisamemberofthepolycombgroup(PcG)familywhichformmultimericproteincomplexesandareinvolvedinmaintainingthetranscriptionalrepressivestateofgenesoversuccessivecellgenerations.TheEZH2activityisdependentontheassociationwithothercomponentsofthePRC2complex(EED,SUZ12/JJAZ1,RBBP4andRBBP7).EZH2mayplayaroleinthehematopoieticandcentralnervoussystems.Over–expressionofEZH2isobservedduringadvancedstagesofprostatecancerandbreastcancer.
Description:
MonoclonalantibodyraisedinmouseagainstthecentralpartofthehumanEZH2protein(Enhancerofzestehomolog2)
Synonym(s):
ENX–1,KMT6
AssayConditions:
ChIPresultsobtainedwiththemonoclonalantibodydirectedagainstEZH2
ChIPassayswereperformedusing2.5µgofthemonoclonalantibodyagainstEZH2(Cat.#25222)onshearedchromatinfrom5millionE14mouseembryonicstemandfromSUZ12-/-cells,usedasanegativecontrol.IgGwasusedasnegativeIPcontrol.QuantitativePCRwasperformedwithprimersspecificfortheHoxA10geneandfortheTgenewhichencodestheBrachyurytranscriptionfactor.Figure1showstherecovery,expressedasa%ofinput(therelativeamountofimmunoprecipitatedDNAcomparedtoinputDNAafterqPCRanalysis).
Concentration:
50µg/50µl
Formulation:
PBScontaining0.05%azide
SpeciesReactivity:
Human,mouse
Purification:
ProteinGpurified
Immunogen:
recombinantprotein
Format:
Aqueousbuffersolution
Storage/StABIlity:
Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.
Application(s):
ChIP(2.5µg/ChIP)
Notes:
TheoptimalantibodyamountperIPshouldbedeterminedbytheend–user.Werecommendtesting1–5µgperIP
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Antibodies
ProductType:
Antibody
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
