Bpsbioscience/Anti&ndash；ERα单克隆抗体/25221/100µl
商品编号：
25221
品牌：
bpsbioscience
市场价：
¥7500.00
美元价：
4500.00
产品分类：
单克隆抗体
公司分类：
monoclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
HostSpecies:Mouse
Clonality:monoclonal
Background:
Theestrogenreceptoralpha(ERalpha,UniProt/Swiss–ProtentryP03372)belongstothefamilyofnuclearhormonereceptors,whichareligand–activatedtranscriptionfactors.Theyareimportantfortheregulationofgeneexpression,cellularproliferationanddifferentiation,sexualdevelopmentandreproductivefunction.Estrogenreceptorsarealsoinvolvedinpathologicalprocessessuchasbreastcancer,andosteoporosis.ERalphacanregulatetranscriptionbydirectbindingtoestrogenresponseelements(EREs)intheDNAorbyinteractionwithothertranscriptionfactors.ItmayalsoformaheterodimerwithERbeta.
Description:
MonoclonalantibodyraisedinmouseagainsthumanERalpha(estrogenreceptoralpha),usingasyntheticpeptide
Synonym(s):
ESR,ESR1,ESRA,NR3A14
AssayConditions:
ChIP-seqresultsobtainedwiththemonoclonalantibodydirectedagainstERalpha
ChIPwasperformedwiththemonoclonalantibodyagainstERalpha(Cat.#25221)onshearedchromatinfromMCF7cellstreatedfor1hourwithestrADIol.TheimmunoprecipitatedDNAwasanalyzedwithanIlluminaGenomeAnalyzer.Librarypreparation,clustergenerationandsequencingwereperformedaccordingtothemanufacturer"sinstructions.The36bptagswerealignedtothehumangenomeusingtheELANDalgorithm.Figure1showstheobtainedpeaksneartheTFF1geneonchromosome21(figure1A),theGREB1andHAAOgenesonchromosome2(figure1BandC),andtheZNF185geneontheX-chromosome(figure1D).
Concentration:
100µl
Formulation:
Ascitesfluidfrommousecontaining0.05%azide
SpeciesReactivity:
Human
CrossReactivity:
Doesnotreactwithchicken;otherspeciesnottested
Purification:
Ascitesfluid
Immunogen:
syntheticpeptide
Format:
aqueoussolution
Storage/StABIlity:
Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.
Application(s):
ChIP/ChIP–seq(2.5µl)
ELISA(1:500–1:5000)
WB(1:500–1:5000)
GelSupershift(1:10–1:20)
Immunochemistry(1:500–1:5000)
IP(1:200–1:5000)
ELISA(1:500–1:5000)
WB(1:500–1:5000)
GelSupershift(1:10–1:20)
Immunochemistry(1:500–1:5000)
IP(1:200–1:5000)
Notes:
TheoptimalantibodyamountperIPshouldbedeterminedbytheend–user.Werecommendtesting1–5µgperIP
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Antibodies
ProductType:
Antibody
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
