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4000-520-616
当前位置: 首页 > 产品中心 > Polyclonal_antibody > Bpsbioscience/Anti–EHMT1 polyclonal antibody/25220/100 µl
商品详细Bpsbioscience/Anti–EHMT1 polyclonal antibody/25220/100 µl
Bpsbioscience/Anti–EHMT1 polyclonal antibody/25220/100 µl
Bpsbioscience/Anti–EHMT1 polyclonal antibody/25220/100 µl
商品编号: 25220
品牌: bpsbioscience
市场价: ¥5800.00
美元价: 3480.00
产地: 美国(厂家直采)
公司:
产品分类: 多克隆抗体
公司分类: Polyclonal_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
HostSpecies:Rabbit
Clonality:polyclonal
Background:
EHMT1(UniProt/Swiss–ProtentryQ9H9B1)isahistonemethyltransferasewhichspecificallymethylateshistoneH3onlysine9.H3K9methylationrepresentsaMarkerforepigenetictranscriptionalrepression.ThisrepressionisestablishedbyrecruitingHP1proteinstothemethylatedhistone.H3K9methylationmaycontributetosilencingofMYCandE2Fresponsivegenesandplaysaroleinmurineembryogenesis.
Description:
PolyclonalantibodyraisedinrabbitagainstmouseEhmt1(euchromatichistone–lysineN–methyltransferase1),usingthreeKLH–conjugatedsyntheticpeptidescontainingasequencefromthecentralpartoftheprotein
Synonym(s):
GLP1,KMT1D
AssayConditions:

Determinationofthetiter
Todeterminethetiter,anELISAwasperformedusingaserialdilutionoftheantibodydirectedagainstmouseEHMT1(Cat.No.25220).Theplateswerecoatedwithamixofthepeptidesusedforimmunizationoftherabbit.Byplottingtheabsorbanceagainsttheantibodydilution(Figure1),thetiteroftheantibodywasestimatedtobe1:10,600.

WesternblotanalysisusingtheantibodydirectedagainstEHMT1
NuclearextractsofHeLacells(40μg)wereanalysedbyWesternblotusingtheantibodyagainstEHMT1(Cat.No.25220)diluted1:1,000inTBS-Tweencontaining5%skimmedmilk.Thepositionoftheproteinofinterest(expectedsize:138kDa)isindicatedontheright;themarker(inkDa)isshownontheleft.

Concentration:
100µl
Formulation:
Wholeantiserumfromrabbitcontaining0.05%azide
SpeciesReactivity:
Human,mouse
Purification:
Wholeserum
Immunogen:
syntheticpeptide
Format:
aqueoussolution
Storage/StABIlity:

Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.

Application(s):
ELISA(1:100–1:200)
WB(1:500)
Notes:
Theoptimalantibodyconcentrationshouldbedeterminedbytheend–user.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Antibodies
ProductType:
Antibody
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。