Bpsbioscience/Anti&ndash；DNMT1多克隆抗体/25216/100µl-免疫在线蚂蚁淘旗下平台

当前位置：首页 > 产品中心 > Polyclonal_antibody > Bpsbioscience/Anti&ndash；DNMT1多克隆抗体/25216/100µl

商品详细Bpsbioscience/Anti&ndash；DNMT1多克隆抗体/25216/100µl

Bpsbioscience/Anti&ndash；DNMT1多克隆抗体/25216/100µl

商品编号： 25216

品牌： bpsbioscience

市场价： ¥5800.00

美元价： 3480.00

产地：美国(厂家直采)

公司：

产品分类：多克隆抗体

公司分类： Polyclonal_antibody

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

立即询价

商品介绍

HostSpecies:Rabbit

Clonality:polyclonal

Background:

DNMT1(UniProt/Swiss–ProtentryP26358)preferentiallymethylatesCpGresiduesinhemimethylatedDNAandisresponsIBLeformaintainingmethylationpatternsestablishedindevelopment.InactivationofDNMT1causesglobaldemethylationandembryoniclethality.DNAmethylationiscoordinatedwithmethylationofhistones.TranscriptionalrepressionbyDNMT1ismediatedbydirectinteractionwithHDAC2

Description:

PolyclonalantibodyraisedinrabbitagainstmouseDNMT1(DNA(cytosine–5)–methyltransferase1),usingaKLH–conjugatedsyntheticpeptidecontaininganaminoacidsequencefromtheC–terminalpartoftheprotein

Synonym(s):

DNMT,MCMT,CXXC9,AIM

AssayConditions:

Determinationofthetiter
Todeterminethetiter,anELISAwasperformedusingaserialdilutionoftheantibodydirectedagainstmouseDNMT1(Cat.No.25216).Theplateswerecoatedwiththepeptideusedforimmunizationoftherabbit.Byplottingtheabsorbanceagainsttheantibodydilution(Figure1),thetiteroftheantibodywasestimatedtobe1:9,600.

WesternblotanalysisusingtheantibodydirectedagainstDNMT1
Westernblotwasperformedonwholecelllysatesfrommousefibroblasts(NIH3T3)andembryonicstemcells(E14Tg2a)withtheantibodyagainstmouseDNMT1(Cat.No.25216),diluted1:500inBSA/PBS-Tween.ThemolecularweightMarker(inkDa)isshownontheleft;thelocationoftheproteinofinterest(expectedsize:183kDa)isindicatedontheright.

Concentration:

100µl

Formulation:

Wholeantiserumfromrabbitcontaining0.05%azide

SpeciesReactivity:

Mouse

Purification:

Wholeserum

Immunogen:

syntheticpeptide

Format:

aqueoussolution

Storage/StABIlity:

Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.

Application(s):

ELISA(1:100–1:1000)
WB(1:500)

Notes:

Theoptimalantibodyconcentrationshouldbedeterminedbytheend–user.

Warning(s):

Avoidfreeze/thawcycles

ScientificCategory:

Antibodies

ProductType:

Antibody

Datashownislot-specific.Contactusforspecificinformationonotherlots.

品牌介绍

bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。

公司介绍

联络我们

服务热线：4000-520-616

（限工作日9:00-18:00）

QQ ：1570468124

手机：18915418616

官网：http://www.bpsbioscience.com