Determinationoftheantibodytiter
Todeterminethetiter,anELISAwasperformedusingaserialdilutionoftheantibodydirectedagainstCre(cat.No.25215)inantigencoatedwells.Byplottingtheabsorbanceagainsttheantibodydilution(Figure1),thetiteroftheantibodywasestimatedtobe1:2,900.
WesternblotanalysisusingtheantibodydirectedagainstCre(1)
Westernblotwasperformedonwholecelllysatesfromuntransfected293cells(lane1),or293cellstransfectedwithCre(lane2),Dre(lane3)orFlp(lane4)withtheantibodyagainstCre(cat.No.25215),diluted1:500inBSA/PBS-Tween.ThemolecularweightMarker(inkDa)isshownontheleft;thelocationoftheproteinofinterest(expectedsize:38kDa)isindicatedontheright.
Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.
WB(1:500)
