
Determinationofthetiter
Todeterminethetiter,anELISAwasperformedusingaserialdilutionoftheantibodydirectedagainstmouseBrd2(Cat.#25211).Thewellswerecoatedwiththepeptideusedforimmunisationoftherabbit.Byplottingtheabsorbanceagainsttheantibodydilution(Figure1),thetiteroftheantibodywasestimatedtobe1:5,000.
WesternblotanalysisusingtheantibodydirectedagainstBrd2
Westernblotwasperformedonwholecelllysatesfrommousefibroblasts(NIH3T3)andembryonicstemcells(E14Tg2a)withtheantibodyagainstmouseBrd2(Cat.#25211),diluted1:1,000inBSA/PBS-Tween.ThemolecularweightMarker(M,inkDa)isshownontheleft;thelocationoftheproteinofinterest(predictedsize:88kDa)isindicatedontheright.
Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.
WB(1:1000)