
WesternblotanalysisusingtheantibodydirectedagainstASH2
Figure1A:WesternblotwasperformedusingtheantibodyagainsthASH2(cat.#25209)diluted1:1,000inTBS-Tweencontaining5%skimmedmilkonnuclearextractsfromHeLacells(upperpanel:lane1)andectopicallyexpressedFLAG-taggedASH2(ASH2-F,upperpanel:lane2).Thelowerpanelshowsthesamewesternblotwithananti-FLAGantibody.ThemolecularweightMarkerisshownontheleft.
Figure1B:WesternblotwasperformedusingnuclearextractsfromHeLacells(HeLaNE,20μg)withtheantibodyagainstASH2(cat.#25209)diluted1:1,000inTBS-Tweencontaining5%skimmedmilk.Themolecularweightmarker(inkDa)isshownontheleft;thelocationoftheproteinofinterestisindicatedontheright.
Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.