Bpsbioscience/Anti&ndash；5&ndash；hmC多克隆抗体（兔）/25204/20µl-免疫在线蚂蚁淘旗下平台

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Bpsbioscience/Anti&ndash；5&ndash；hmC多克隆抗体（兔）/25204/20µl

商品编号： 25204

品牌： bpsbioscience

市场价： ¥4300.00

美元价： 2580.00

产地：美国(厂家直采)

公司：

产品分类：多克隆抗体

公司分类： Polyclonal_antibody

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

HostSpecies:Rabbit

Clonality:polyclonal

Background:

5–hydroxymethylcytosine(5–hmC)resultsfromtheenzymaticconversionof5–methylcytosineinto5–hydroxymethylcytosinebytheTETfamilyofiron–dependentoxygenases.5–hmCbaseswererecentlydiscoveredinmammalianDNA,inPurkinjeneurons,ingranulecellsandembryonicstemcellswheretheyarepresentathighlevels(upto0.6%oftotalnucleotidesinPurkinjecells).Recentreportsindicatethat5–hmCisabundantinbraintissue,especiallyinareasthatareassociatedwithhighercognitivefunctions.Preliminaryresultsindicatethat5–hmCmayhaveimportantrolesdistinctfrom5–mC.Althoughitspreciserolehasstilltobeshown,earlyevidencesuggests5–hydroxymethylcytosinemayrepresentanewanduniquepathwaytodemethylateDNAinvolvingarepairmechanismconverting5–hmCtocytosine.Duetothestructuralsimilaritybetween5–mCand5–hmC,thesebasesareexperimentallyalmostindistinguishable.Themostcommonlyusedmethodologies(e.g.enzymaticapproaches,bisulfitesequencing)donotdistinguish5mcfrom5–hmC.Thedevelopmentofspecificantibodiesappearstobethemostpowerfulwaytodistinguishandspecificallyenrichfor5–mCand5–hmCsequences.

Description:

Polyclonalantibodyraisedinrabbitagainst5–hydroxymethylcytosine(5–hmC)conjugatedtoKLH

AssayConditions:

Determinationofthe5-hmCrabbitpolyclonalantibodytiter
Todeterminethetiter,anELISAwasperformedusingaserialdilutionoftherabbitpolyclonalantibodydirectedagainst5-hmCinantigencoatedwells.TheantigenusedwasBSAcoupledtothe5-hmCbase.Byplottingtheabsorbanceagainsttheantibodydilution,thetiteroftheantibodywasestimatedtobe1:3,500.

AnhydroxymethylatedDNAIP(hMeDIP)wasperformedusingtherabbitpolyclonalantibodydirectedagainst5-hydroxymethylcytosine
TheIgGisotypeantibodiesfromrabbit(Cat.No.25204)wasusedasnegativecontrol.TheDNAwaspreparedwiththeGenDNAmoduleofthehMeDIPkitandsonicatedtohaveDNAfragmentsof300-500bp.1μgofhumanHeLacellsDNAwerespikedwithnon-methylated,methylated,andhydroxymethylatedfragments.TheimmunoprecipitatedmaterialhasbeenanalysedbyqPCRusingtheprimerpairspecificforthe3differentcontrolsequences.Theobtainedresultsshowthattherabbitpolyclonalfor5-hmCishighlyspecificforthisbasemodification(noIPwithnon-methylatedormethylatedCbasescontainingfragments).

Dotblotanalysisofthe5-hmCrabbitpolyclonalantibodywiththeC,mCandhmCPCRcontrols
100to4ng(equivalentof5to0.2pmolofC-bases)ofthehmC,mCandCPCRcontrolswerespottedonamembrane(AmershamHybond-N+).Themembranewasincubatedwiththerabbit5-hydroxymethylcytosinepolyclonalantibody(cat.no.25204,dilution1:200).Themembraneswereexposedfor30seconds.

Concentration:

20µl

Formulation:

Wholeantiserumfromrabbitcontaining0.05%azide

SpeciesReactivity:

Human,mouse,broadrange

Purification:

Wholeserum

Immunogen:

nucleotide

Format:

aqueoussolution

Storage/StABIlity:

Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.

Application(s):

hMeDIP(2.5µg/IP)
ELISA(1:500)
DB(1:200)

Notes:

Theoptimalantibodyconcentrationshouldbedeterminedbytheend–user.

Warning(s):

Avoidfreeze/thawcycles

ScientificCategory:

Antibodies

ProductType:

Antibody

Datashownislot-specific.Contactusforspecificinformationonotherlots.

品牌介绍

bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。

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