Bpsbioscience/Anti&ndash；5&ndash；hmC单克隆抗体（小鼠）/25202/20µg-免疫在线蚂蚁淘旗下平台

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Bpsbioscience/Anti&ndash；5&ndash；hmC单克隆抗体（小鼠）/25202/20µg

商品编号： 25202

品牌： bpsbioscience

市场价： ¥4300.00

美元价： 2580.00

产地：美国(厂家直采)

公司：

产品分类：单克隆抗体

公司分类： monoclonal_antibody

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

HostSpecies:Mouse

Clonality:monoclonal

Background:

5–hydroxymethylcytosine(5–hmC)resultsfromtheenzymaticconversionof5–methylcytosineinto5–hydroxymethylcytosinebytheTETfamilyofiron–dependentoxygenases.5–hmCbaseswererecentlydiscoveredinmammalianDNA,inPurkinjeneurons,ingranulecellsandembryonicstemcellswheretheyarepresentathighlevels(upto0.6%oftotalnucleotidesinPurkinjecells).Recentreportsindicatethat5–hmCisabundantinbraintissue,especiallyinareasthatareassociatedwithhighercognitivefunctions.Preliminaryresultsindicatethat5–hmCmayhaveimportantrolesdistinctfrom5–mC.Althoughitspreciserolehasstilltobeshown,earlyevidencesuggests5–hydroxymethylcytosinemayrepresentanewanduniquepathwaytodemethylateDNAinvolvingarepairmechanismconverting5–hmCtocytosine.Duetothestructuralsimilaritybetween5–mCand5–hmC,thesebasesareexperimentallyalmostindistinguishable.Themostcommonlyusedmethodologies(e.g.enzymaticapproaches,bisulfitesequencing)donotdistinguish5mcfrom5–hmC.Thedevelopmentofspecificantibodiesappearstobethemostpowerfulwaytodistinguishandspecificallyenrichfor5–mCand5–hmCsequences.

Description:

Monoclonalantibodyraisedinmouseagainst5–hydroxymethylcytosineconjugatedtoBSA

AssayConditions:

AnhydroxymethylatedDNAIP(hMeDIP)wasperformedusingthemousemonoclonalantibodydirectedagainst5-hydroxymethylcytosine(Cat.#25202).
TheIgGisotypeantibodiesfrommousewereusedasnegativecontrol.TheDNAwaspreparedwiththeGenDNAmoduleofthehMeDIPkitandsonicatedtohaveDNAfragmentsof300-500bp.1μgofhumanHeLacellsDNAwerespikedwithnon-methylated,methylated,andhydroxymethylatedPCRfragments.TheimmunoprecipitatedmaterialwasanalyzedbyqPCRusingtheprimerpairspecificforthe3differentcontrolsequences.Theobtainedresultsshowthatthemousemonoclonalfor5-hmCishighlyspecificforthisbasemodification(noIPwithnon-methylatedormethylatedCbasescontainingfragments).

Determinationofthe5-hmCmousemonoclonalantibodytiter
Todeterminethetiter,anELISAwasperformedusingaserialdilutionofthemousemonoclonalantibodydirectedagainst5-hmC(Cat.#25202)inantigencoatedwells.TheantigenusedwasKHLcoupledto5-hmCbase.Byplottingtheabsorbanceagainsttheantibodydilution,thetiteroftheantibodywasestimatedtobe1:40,000.

Dotblotanalysisofthe5-hmCmousemonoclonalantibodywiththeC,mCandhmCPCRcontrols
200to2ng(equivalentof10to0.1pmolofC-bases)ofthehmC(1),mC(2)andC(3)PCRcontrolsfrom5-hmC,5-mC&cytosineDNAwerespottedonamembrane(AmershamHybond-N+).Themembranewasincubatedwith2μg/mlofthemouse5-hydroxymethylcytosinemonoclonalantibody(dilution1:500).Themembraneswereexposedfor30seconds.

Concentration:

20µg/20µl

Formulation:

PBS(ph7.4)containing0.05%sodiumazide

SpeciesReactivity:

Human,mouse,broadrange

CrossReactivity:

Specificfor5–hmc.Doesnotreactwith5–mcorunmodifiedcytosine.

Purification:

ProteinA/Gpurified

Immunogen:

nucleotide

Format:

Aqueousbuffersolution

Storage/StABIlity:

Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.

Application(s):

hMeDIP(2.5µg/IP)
DB(2µg/ml)
ELISA(1:500)
IF

Notes:

Theoptimalantibodyconcentrationshouldbedeterminedbytheend–user.

Warning(s):

Avoidfreeze/thawcycles

ScientificCategory:

Antibodies

ProductType:

Antibody

Datashownislot-specific.Contactusforspecificinformationonotherlots.

品牌介绍

bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。

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