Bpsbioscience/Anti&ndash；5&ndash；caC多克隆抗体/25201/20µg-免疫在线蚂蚁淘旗下平台

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商品详细Bpsbioscience/Anti&ndash；5&ndash；caC多克隆抗体/25201/20µg

Bpsbioscience/Anti&ndash；5&ndash；caC多克隆抗体/25201/20µg

商品编号： 25201

品牌： bpsbioscience

市场价： ¥4300.00

美元价： 2580.00

产地：美国(厂家直采)

公司：

产品分类：多克隆抗体

公司分类： Polyclonal_antibody

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

HostSpecies:Rabbit

Clonality:polyclonal

Background:

Theso-called6thbase,5–hydroxymethylcytosinehasbeendiscoveredin2009.Thefunctionofthisnewmodifiedbaseremainsmostlyunclear,butitiscertainthatthe5–hmCbaseisgeneratedbyenzymaticconversionof5–methylcytosine(5–mC)into5–hydroxymethylcytosinebytheTETfamilyofoxygenases.Earlyreportssuggestedthat5–hmCmayrepresentanintermediateofactivedemethylationinanewpathwaywhichdemethylatesDNA,converting5–mCtocytosine.Recentevidencefuelthehypothesisthat5–hmCrepresentsanintermediate,whichcouldinvolvefurtheroxidationofthehydroxymethylgrouptoaformylorcarboxylgroupfollowedbyeitherdeformylationordecarboxylation.Thecarboxylandformylgroupsof5–formylcytosine(5–fC)and5–carboxylcytosine(5–caC)couldbeenzymaticallyremovedwithoutexcisionofthebase.

Description:

Polyclonalantibodyraisedinrabbitagainst5–Carboxylcytosine(5ca–CMPmonophosphate)conjugatedtoBSA

Synonym(s):

5–Carboxylcytosine

AssayConditions:

Dotblotanalysisusingtheantibodydirectedagainst5-caC
Todemonstratethespecificityoftheantibodyagainst5-caC(Cat.#25201),aDotBlotanalysiswasperformedusingsyntheticoligonucleotidescontainingdifferentmodifiedC-bases(indicatedinred).125and25ngoftherespectiveoligoswereboundtoaStreptavindin-coatedmulti-wellplate.Theantibodywasusedatadilutionof1:1,000.ThebindingofantibodytotheDNAwasmeasuredbyECLchemiluminescence.Figure1showsahighspecificityoftheantibodyforthecarboxylatedcytosine.

Immunofluorescenceassayusingtheantibodydirectedagainst5-caC
293TcellsweretransfectedwitheitherthemouseFLAG-taggedwild-typeTet1(Tet1CD)orthecatalyticallyinactiveFLAG-taggedC-terminaldomainofTet1(Tet1mCD)andstainedwiththeantibodyagainst5-caC(Cat.#25201),diluted1:500,andwithananti-FLAGantibody,followedbyDAPIcounterstaining.

Immunoprecipitationusingtheantibodydirectedagainst5-caC
Immunoprecipitationwasperformedwiththeantibodyagainst5-caC(Cat.#25201)on2µgofJ1ESgenomicDNA,spikedwith1pgofacontrolDNAfragment(approximately700bpfromtheRFP(Ringfingerprotein)gene)containingdifferentcytosinemodifications.ThemCandhmCcontrolDNAwasgeneratedbyPCRwiththecorrespondingnucleotide.ThecaCcontrolfragmentwasobtainedbyinvitromethylationusingM.SssImethyltransferasefollowedbyoxidationwithpurifiedTet2.TheimmunoprecipitatedDNAwassubsequentlyanaysedbyqPCRusingprimersspecificforthecontrolDNAfragmentsandforGAPDH,usedasanegativecontrol.Figure3showstheenrichmentcalculatedastheratiooftherecoveryofthecontrolDNAversustherecoveryoftheGAPDHnegativecontrol.

Concentration:

20µg

Formulation:

PBS(pH7.4),30%glycerol,0.05%sodiumazide

SpeciesReactivity:

CrossReactivity:

Specificfor5–carboxylcytosine(5–caC).Doesnotreactwith5–mc,5–hmc,unmodifiedcytosine,or5–formylcytosine(5–fC)and5–carboxylcytosine(5–caC)

Purification:

ProteinAaffinitypurified

Immunogen:

nucleotide

Format:

Aqueousbuffersolution

Storage/StABIlity:

Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.

Application(s):

DB(1:500–1:1000)
IF(1:500)
DNAIP(4µggenomicDNA/IP)

Notes:

Theoptimalantibodyconcentrationshouldbedeterminedbytheend–user.

Warning(s):

Avoidfreeze/thawcycles

ScientificCategory:

Antibodies

ProductType:

Antibody

Datashownislot-specific.Contactusforspecificinformationonotherlots.

品牌介绍

bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。

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