Bpsbioscience/Anti&ndash；3&ndash；mC多克隆抗体/25200/50µg
商品编号：
25200
品牌：
bpsbioscience
市场价：
¥7200.00
美元价：
4320.00
产品分类：
多克隆抗体
公司分类：
Polyclonal_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
HostSpecies:Rabbit
Clonality:polyclonal
Background:
3–methylcytosinearisesbyerroneousmethylationofDNAthroughspontaneousexposuretoendogenousS–adenosylmethionine(SAM).SincetheN3–positionincytosineisprotectedindoublestrandedDNA,thisphenomenonmainlyoccursinsinglestrandedDNA.Theresulting3–mCistoxicandmutagenicandincreasedlevelsof3–mCseemtoimpaircellproliferation.Therefore,ithastoberepairedbythecell.Thisisestablishedbyoxidativedemethylation,whichiscatalyzedbytheAlkBprotein.
Description:
Polyclonalantibodyraisedinrabbitagainst3–methylcytosine(3–mC)conjugatedtoBSA.
AssayConditions:
Dotblotanalysisusingtheantibodydirectedagainst3-mC
Todemonstratethespecificityoftheantibodyagainst3-mC(cat.No.25200),aDotBlotanalysiswasperformedusingsyntheticoligonucleotidescontainingdifferentmodifiedbases.150and50pmoloftherespectiveoligo’swerespottedonthemembrane.Theantibodywasdiluted1:400inPBS-Tcontaining10%skimmedmilkand1%BSA.Figure1showsahighspecificityoftheantibodyfortheoligonucleotidewiththe3-methylcytosinemodification.
Concentration:
50µg/28µl
Formulation:
PBScontaining0.05%azideand0.05%ProClin300.
SpeciesReactivity:
Human,mouse,broadrange
CrossReactivity:
Doesnotcross–reactwith5–methylcytosine,5–hydroxymethylcytosine,or5–carboxylcytosine.
Purification:
ProteinGpurified
Immunogen:
syntheticpeptide
Format:
Aqueousbuffersolution
Storage/StABIlity:
Storeat–80°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat–20°Cforatleastonemonth.
Application(s):
DB(1:400)
Notes:
Theoptimalantibodyconcentrationshouldbedeterminedbytheend–user.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Antibodies
ProductType:
Antibody
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
