Bpsbioscience/P300 Chemiluminescent Assay Kit/50077L/96 reactions
商品编号：
50077L
品牌：
bpsbioscience
市场价：
¥8700.00
美元价：
5220.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Background:
TheadenovirusE1A-associatedcellularp300transcriptionalco-activatorproteinfunctionsasahistoneacetyltransferasethatregulatestranscriptionviachromatinremodelingandisimportantintheprocessesofcellproliferationanddifferentiation.p300(alsoknownasKAT3B)acetylatesallfourcorehistonesinnucleosomes,aswellasnon-histonetargets.p300mediatescAMP-generegulationbybindingspecificallytophosphorylatedCREBprotein.p300hasalsobeenidentifiedasaco-activatorofHIF1A(hypoxia-inducIBLefactor1alpha),whichplaysaroleinthestimulationofhypoxia-inducedgenessuchasVEGF.DefectsinthisgeneareacauseofRubinstein-TaybisyndromeanditsdysfunctionislinkedtoanumberofhumandiseasesincludingParkinson’s,Huntington’s,andAlzheimer’sdiseases.p300mayalsoplayaroleinepithelialcancer.
Description:
Thep300ChemiluminescentAssayKitisanenzyme-linkedimmunosorbentassay(ELISA)designedtoscreenforinhibitorsofp300.TheP300ChemiluminescentAssayKitisbasedonthep300enzymetransferringanacetylgroupfromanacetyldonor(acetylCoA)toahistonesubstrate.Theacetylatedhistoneisrecognizedbyahighlyspecificprimaryantibody,followedbyanHRP-labeledsecondaryantibody.ThechemiluminescenceproducedbyHRPcanbemeasuredusingachemiluminescencereader.
UniProt
Q09472
Synonym(s):
EP300,KAT3B,RSTS2,E1Abindingprotein
SuppliedAs:
Kitcomesinaconvenientformat,witha96-wellplateprecoatedwithNeutravidinandHistonepeptide,andallthereagentsnecessaryfor96chemiluminescentP300activitymeasurements.Inaddition,thekitincludespurifiedp300foruseasapositivecon
Contraindications:
DMSO>1%,strongacidsorbases,ionicdetergents,highsalt.
Format:
COMPONENTS:
Storage/StABIlity:
Stableatleast6monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.
Application(s):
GreatforstudyingenzymeactivityandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.
Reference(s):
Ravindra,K.C.,etal.(2012).ChemResToxicol.25(2):337–347.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Acetyltransferase
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
